Materials and Methods: Aerial parts of plant in blooming were collected from Deraznoo mountain (2500 m) in August 2013, ecological and traditional data were recorded. The aqueous and methanol extracts were isolated by maceration, antioxidant capacity were measured by total antioxidant capacity (TAC), reducing power (RP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) in comparison with butylated hydroxy toluene (BHT) and butylated hydroxyanisole BHA antioxidant standard and then their antibacterial activity were studied in vitro against 9 gram positive and negative bacteria by using well method and the minimum inhibitory concentration (MIC) assay.

Results: Ziziphora clinopodioides Lam. (Z. clinopodioides Lam) often grows in sunny environment in Deraznoo mountain (2500 m), with annual rainfall of 334 mm and annual temperature of 10.3°C in temperate cold climate and sandy loam soil with Ec = 0.6 and pH = 6.9. It has been used by the rural people as an antispasm, anti-inflammatory, antifungal , anti-infective, sedative and expectorant agent to treat cold, flu, diarrhea, gasterointestinal disorder and stomach ache. The ethanol extract of plant had high antioxidant activity with IC50 values (32.5 ± 0.4 μg/ml) especially in DPPH assay and exhibited good antibacterial activity on Staphylococcus aureus, Staphylococcus epidermidis, Bacillus cereus and Pseudomonas aeruginosa with IZ (28.1 ± 0.5, 24.8 ± 0.1, 19.4 ± 1.1 and 14.8 ± 1 mm), respectively with high MIC value of 14.5 μg/ml, respectively.

Conclusion: The methanol extract of Z. clinopodioides Lam. have suitable antibacterial and antioxidant activity which can be used as natural anti-infective agent to treat many infectious diseases.