|In Vitro Study of Berberis vulgaris, Actinidia deliciosa and Allium cepa L. Antibacterial Effects on Listeria monocytogenes|
|Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran|
CJMB 2015; 2: 111-115
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Keywords : Actinidia, Anti-bacterial agents, Listeria monocytogenes, Onions
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Objective: One control method of pathogenic microorganisms is using synthetic chemical preservatives and antibiotics. Because of being generally recognized as safe, antibacterial compounds with organic origin are considered important for health. This study was done in order to investigate the antibacterial effects of methanol extracts of the Berberis vulgaris (Barberry), Actinidia deliciosa (Kiwi) and Allium cepa L. (Onions) on the standard strain (ATCC:19114) of Listeria monocytogenes (L. monocytogenes), as it seems that it is possible to find some important organic and health safe anti-Listerial compounds.
Materials and Methods: After collecting the mentioned plants phytology study was done. Then methanol extracts of named plants were prepared and antibacterial effects of these plants against the mentioned strain of L. monocytogenes by the Disk Diffusion, minimum inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) methods were performed. Also Ampicillin (10 μg/disc) was used as the reference antibacterial substance. In order to find the relationship between antibacterial properties of plants extracts independent t test, chi-square and analysis of variance (ANOVA) tests were used.
Results: Results showed that the extracts of all the three studied plants had antibacterial effects on L. monocytogenes. Average diagonal of growing area in disk diffusion test for barberry, kiwi and onions in order was 12, 15.5 and 11 mm. Also MIC of mentioned plants extracts in order was 125, 62.5, and 125 μg/ml and MBC of named plants was 500, 250 and 500 μg/ ml, respectively.
Conclusion: The results of this work showed that methanol extracts of kiwi had stronger anti-Listerial effect than barberry’s and onion’s extracts.