Original Article | |
Biosynthesis and Characterization of Silver Nanoparticles Using the Sewage of a Leather Factory in Corn Steep Liquor | |
Naeimeh Faridi Aghdam1, Shahram Moradi1, Sirous Ebrahimi2, Hamed Hamishehkar3 | |
1Faculty of Chemistry, Islamic Azad University, North Tehran Branch, Tehran, Iran 2Biotechnology Research Centre, Faculty of Chemical Engineering, Sahand University of Technology, Tabriz, Iran 3Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
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CJMB 2024; 11: 092-097 DOI: 10.34172/cjmb.2023.4012 Viewed : 1568 times Downloaded : 1306 times. Keywords : Silver nanoparticles, Biosynthesis, Sewage, Leather factory, Corn steep liquor |
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Abstract | |
Objectives: This study aimed to report a simple environmentally compatible method that is economically affordable for the facile biosynthesis of stable silver nanoparticles (AgNPs) in corn steep liquor (CSL) nutrient using the sewage of a leather factory. Materials and Methods: The biosynthesis of AgNPs were done by reduction of AgNO3 in the sewage of leather factory as a mixed bacterial culture and corn steep liquor under anaerobic condition while protecting from light. Results: The AgNPs were found to have a characteristic absorption peak at 416 nm on the ultraviolet-visible spectrum. Characterization of AgNPs was performed by x-ray diffraction (XRD) and Fourier-transform infrared (FTIR). The scanning electron microscopy (SEM) and transmission electron microscopes (TEM) images showed poly-dispersed spherical stable AgNPs with a maximum diameter of 20 nm. Moreover, the dynamic light scattering (DLS) showed that the average size of AgNPs was 15.57 nm with a zeta potential value of -19.2 mV. The synthesized nanoparticles demonstrated more antibacterial activity in opposition to gram-negative bacteria. The zone of inhibition of biosynthesized AgNPs in 1 mM concentration of AgNO3 against Escherichia coli was equal to that of gentamicin. Conclusions: The interaction of protein residues with AgNPs was identified, supporting that the proteins not only act as a reducing agent but also as a capping. The synthesized AgNPs showed antibacterial activity, providing commercial viability in biomedicine. |
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