Login Contact Us
E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Apr 2024, Vol 11, Issue 2
Advanced Search
Title
Authors
Keyword
Poll
How do you find the scientific quality of the published articles on our web site?
Original Article
Prevalence of C677T Single Nucleotide Polymorphism of Methylenetetrahydrofolate Reductase Gene and its Relationship With Serum Levels of Homocysteine, Vitamin B12, Folate, and Cholesterol in Alzheimer's Patients
Sanaz Amani1, Ebrahim Mirzajani2,3, Mohammad Taghi Goodarzi4, Amir Reza Ghayeghran5
1Department of Chemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran
2Department of Biochemistry, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
3Cellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
4Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran
5Department of Neurology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

DOI: 10.34172/cjmb.2023.36
Viewed : 470 times
Downloaded : 711 times.

Keywords : Alzheimer
Full Text(PDF) | Related Articles
Abstract
Objectives: Many single nucleotide polymorphisms affect the incidence of Alzheimer's disease (AD). This investigation aimed to consider the frequency of the C677T single nucleotide polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene in Alzheimer's patients.

Materials and Methods: This study was conducted in two groups of control (n = 80) and patient (n = 80) with a ratio of 1: 1 male to female. Amplification-refractory mutation system-Polymerase Chain Reaction (ARMS-PCR) method was used to study mutations and ELISA was used to measure homocysteine and the chemiluminescence method was used to measure cholesterol, vitamin B12 and folate.

Results: Based on the results of the PCR test of the MTHFR gene, the incidence rate of mutation in the healthy allele was 44.6% and in the mutant allele was 27.9% of the total study population.

Conclusion: In this study, it was discovered that an increase in cholesterol levels is related with an increased risk of developing the disease, but more studies are needed to confirm this. It should also be noted that this increase is not related to the MTHFR gene polymorphism at the C677T position.

 

Cite By, Google Scholar

Google Scholar

Articles by Amani S
Articles by Mirzajani E
Articles by Taghi Goodarzi M
Articles by Reza Ghayeghran A

PubMed

Articles by Sanaz Amani
Articles by Ebrahim Mirzajani

Submit Paper
Online Submission System
CJMB ENDNOTE ® Style Tutorials Publication Charge Women's Reproductive Health Research Center About Journal
Publication Information
Publisher
Aras Part Medical International Press Editor-in-Chief
Arash Khaki
Deputy Editor
Zafer Akan
Published Article Statistics