Prevalence of C677T Single Nucleotide Polymorphism of Methylenetetrahydrofolate Reductase Gene and its Relationship With Serum Levels of Homocysteine, Vitamin B12, Folate, and Cholesterol in Alzheimer's Patients

Sanaz Amani; Ebrahim Mirzajani; Mohammad Taghi Goodarzi; Amir Reza Ghayeghran

doi:10.34172/cjmb.2023.36

E-ISSN : 2148-9696

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Original Article
Prevalence of C677T Single Nucleotide Polymorphism of Methylenetetrahydrofolate Reductase Gene and its Relationship With Serum Levels of Homocysteine, Vitamin B12, Folate, and Cholesterol in Alzheimer's Patients
Sanaz Amani¹, Ebrahim Mirzajani^2,3, Mohammad Taghi Goodarzi⁴, Amir Reza Ghayeghran⁵
¹Department of Chemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran ²Department of Biochemistry, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran ³Cellular and Molecular Research Center, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran ⁴Department of Biochemistry, Shahrood Branch, Islamic Azad University, Shahrood, Iran ⁵Department of Neurology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran
DOI: 10.34172/cjmb.2023.36 Viewed : 487 times Downloaded : 734 times. Keywords : Alzheimer
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Abstract

Objectives: Many single nucleotide polymorphisms affect the incidence of Alzheimer's disease (AD). This investigation aimed to consider the frequency of the C677T single nucleotide polymorphism of the methylenetetrahydrofolate reductase (MTHFR) gene in Alzheimer's patients. Materials and Methods: This study was conducted in two groups of control (n = 80) and patient (n = 80) with a ratio of 1: 1 male to female. Amplification-refractory mutation system-Polymerase Chain Reaction (ARMS-PCR) method was used to study mutations and ELISA was used to measure homocysteine and the chemiluminescence method was used to measure cholesterol, vitamin B12 and folate. Results: Based on the results of the PCR test of the MTHFR gene, the incidence rate of mutation in the healthy allele was 44.6% and in the mutant allele was 27.9% of the total study population. Conclusion: In this study, it was discovered that an increase in cholesterol levels is related with an increased risk of developing the disease, but more studies are needed to confirm this. It should also be noted that this increase is not related to the MTHFR gene polymorphism at the C677T position.