Login Contact Us
E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Oct 2022, Vol 9, Issue 4
Advanced Search
How do you find the scientific quality of the published articles on our web site?

Original Article
Evaluation of Testosterone, Blood Antioxidants, and Histopathological Changes Following Chemical Castration With Calcium Chloride in Rats
Mehdi Hami1, Abbas Veshkini1, Alireza Jahandideh1, Siamak Mashhadi Rafiee1, Pejman Mortazavi2
1Department of Clinical Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran

CJMB 2022; 9: 207-212
DOI: 10.34172/cjmb.2022.34
Viewed : 1159 times
Downloaded : 1628 times.

Keywords : Calcium chloride, Chemical castration, Antioxidant, Histopathology, Testosterone, Rat
Full Text(PDF) | Related Articles
Objectives: Castration is one of the most common methods of contraception in animals. This method can prevent animals from contracting various diseases, such as testicular tumors, and prevent the overgrowth of animal populations.

Materials and Methods: In this experiment, 10 adult and clinically healthy male Wistar rats weighing approximately 250-200 g were divided into two equal groups of control and treatment (n=5 in each). In the control group, normal saline and in the treatment group, 20% calcium chloride was injected into the testicular tissue. Blood samples were taken at the beginning and end of the experiment (day 21) and the superoxide dismutase (SOD), glutathione peroxidase (GPx), and testosterone levels were measured. Then, the tissue samples of testis were evaluated on days 7, 14, and 21.

Results: On day 21, SOD and GPx enzymes significantly increased in the treatment group compared to day zero (P = 0.01). However, calcium chloride injection reduced the mean testosterone levels on day 21 compared to day zero (P = 0.06). Meanwhile, the mean number of sperms in the right testis of treated mice significantly decreased (P = 0.03). Seminiferous tubule necrosis significantly increased on day 7, neutrophil infiltration significantly increased on days 7 and 14, and calcification significantly increased on days 7 and 21 (P < 0.01).

Conclusions: Although chemical sterilization using 20% calcium chloride can lead to testicular degeneration, it is not a recommended method because it does not reduce testosterone and activates oxidative stress biomarkers. However, the negative effects might be eliminated in future studies using some substances such as tannic acid.


Cite By, Google Scholar

Google Scholar

Articles by Hami M
Articles by Veshkini A
Articles by Jahandideh A
Articles by Mashhadi Rafiee S
Articles by Mortazavi P


Articles by Mehdi Hami
Articles by Abbas Veshkini
Articles by Alireza Jahandideh
Articles by Pejman Mortazavi

Submit Paper
Online Submission System
CJMB ENDNOTE ® Style Tutorials Publication Charge Women's Reproductive Health Research Center About Journal
Publication Information
Aras Part Medical International Press Editor-in-Chief
Arash Khaki
Deputy Editor
Zafer Akan
Published Article Statistics