|Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line|
|Parviz Faraji1,2, Mostafa Araj-Khodaei1,3, Amir Jafari4, Maryam Ghaffari2, Reza Mohammadinasab5, Sanaz Hamedeyazdan6, Miguel de la Guardia7, Jafar Ezzati Nazhad Dolatabadi8|
|1Department of Persian Medicine, School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3Neurosciences Research Center, Aging Research Institute, Tabriz University of Medical Sciences, Iran
4Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5Department of History of Medicine, School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
6Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
7Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain
8Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
CJMB 2022; 9: 189-194
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Keywords : Methotrexate, Melissa officinalis, HeLa cancer cells, MTT assay, Flow cytometry
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Objectives: One of the well-accepted beliefs about natural products, considering the advances of recently appearing new edges and features of herbal medicine, is paying more attention to cancer treatments. However, they have not been properly studied with reasonable/reliable clinical trials in human subjects in most cases. Therefore, seeking in vitro effects of herbs like Melissa officinalis (MO) in cancer therapy to identify the involved possible mechanism in conjugation with configurative/morphological aspects of treated cells seems quite necessary. In this study, we evaluated the co-treatment effect of anti-cancer drug methotrexate (MTX) and MO on HeLa cancer cells.
Methods: MTT assay was applied to assess the quantitative cytotoxicity effect of both MTX and Mo. Apoptosis assay via flow cytometry was used to determine the amount of apoptotic and necrotic cells. To further investigate the anti-cancer effects, DAPI staining and DNA ladder assays are used qualitatively to detect changes in the nuclei of cells that are a sign of apoptosis occurring and morphological modifications of DNA.
Results: MTX and MO mixture showed high cytotoxicity and apoptosis rate compared to untreated cells. Furthermore, the morphological changes of MTX and MO mixture were more evident than that of single MO, MTX, and control groups.
Conclusions: These data regarding cell growth reduction and apoptosis induction in HeLa cancer cells showed that MTX and MO mixture can be an appropriate platform for cancer therapy.
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