Biological and Antibacterial Features of Acroptilon repens (L.) DC

Zahra Akhgari; Asghar Tanomand; Razieh Nazari; Mohsen Zargar

doi:10.34172/cjmb.2022.20

E-ISSN : 2148-9696

Crescent Journal of
Medical and Biological Sciences

Main Page Aim and Scope Instructions to Authors Editorial Policies Editorial Board Authorship Agreement Form Open Access and Copyright

Apr 2022, Vol 9, Issue 2

CURRENT ISSUE ARCHIVE AHEAD OF PRINT / ACCEPTED ARTICLE MOST VIEWED ARTICLES NEWS RELATED LINKS

Advanced Search

For Author's & Reviewer's

Committee on Publication
Ethics (COPE) Flowchart

Recommendations for the Conduct

World Association of Medical Editors(WAME)

EQUATOR Resources : Reviewers

Poll

How do you find the scientific quality of the published articles on our web site?

Very good

Good

Fair

Bad

Very bad

Original Article
Biological and Antibacterial Features of Acroptilon repens (L.) DC
Zahra Akhgari¹, Asghar Tanomand², Razieh Nazari¹, Mohsen Zargar¹
¹Department of Microbiology, Islamic Azad University, Qom Branch, Qom, Iran ²Department of Microbiology, Maragheh Faculty of Medical Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
CJMB 2022; 9: 116-122 DOI: 10.34172/cjmb.2022.20 Viewed : 1846 times Downloaded : 1995 times. Keywords : Acroptilon repens, Antibacterial, Antibiofilm, MTT assay
Full Text(PDF) \| Related Articles
Abstract

Objectives: Acroptilon repens (L.) DC is an Asian native plant belongs to the Asteraceae family. It has been cultivated the United States and Canada in recent years. This study investigated the effects of antibacterial, anti-biofilm, and cytotoxicity of A. repens ethanolic extract and its components. Materials and Methods: We used disk diffusion and well diffusion strategies to screen antimicrobial activity of A. repens against Staphylococcus aureus and Staphylococcus epidermidis. Then, the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of extract and fractions were determined by the small dilution broth methodology for S. aureus and S. epidermidis. The impact of extract and fractions on biofilm formation was evaluated by microtiter plate assay The MTT assay determined the toxicity effect of samples on Vero cells. The results are expressed because of the viability. Results: Results showed that A. repens extract and fragments inhibit the growth of gram-positive bacteria. The ethanolic extract and its fractions showed stronger inhibition zones for S. aureus and S. epidermidis. According to the antibiofilm test, the ethanolic extract and water fraction of A. repens have the highest antibiofilm effect on S. aureus and S. epidermidis. The value of LC50 (50% mortality) in MTT assay results was 30, 60, 30, and 2/5 mg/mL for ethanolic extract, chloroform, ethyl acetate and water fractions of A. repens, respectively. Conclusions: Extract of A. repens and its fractions have an antibacterial and antibiofilm effect on S. aureus and S. epidermidis without significant cytotoxic effect.