Materials and Methods: A total of 209 premature infants with birth weight less than 1500 g who were suspicious of having infection, were evaluated in 2 groups of control (107 infants) and intervention (102 infants). All of the infants evaluated for sepsis according to the protocol of the unit. In the control group, antibiotics treatment continued as long as the venous line persist, in the intervention group after day 3 to 5 if the results of blood culture were negative, the infants were checked for C-reactive protein (CRP), and if it was negative too and the patient’s clinical status was good, antibiotic treatment was stopped. The outcome measures were short-term prognosis of with VLBW newborns.

Results: The mean gestational age of the studied patients was 30.21 ± 2.69 and 29.57 ± 2.09 g in the control and intervention groups, respectively (P = 0.07). The average days of receiving antibiotics in the control group were 29.21 ± 1.57 while in the intervention group it was 8.11 ± 2.16 (P < 0.001). Our study suggests that duration of hospitalization (P < 0.001), need for mechanical ventilation (P < 0.001) and duration of use of high-flow nasal cannula (HFNC) (P = 0.04), blood products transfusion (P = 0.007), length of stay in hospital (P < 0.001) were dramatically decreased in intervention group comparing to control group. Rate of later sepsis work-ups was decreased as well. But, in other findings such as need for continues positive airway pressure (CPAP), parenteral nutrition, mortality rate, need for resuscitation and rate of urinary tract infection, no meaningful difference were found between two groups (P > 0.05).

Conclusion: Early discontinuing of antibiotics (5 days or less) had no impact on the mortality rate of VLBW infants and seemed it was safe.