The Preventive Role of Low-Level Laser Therapy in Cochlear Outer Hair Cell Damage Due to Noise Exposure in Guinea Pigs

Ahmad Zeinalzade; Ghassem Mohammadkhani; Mehdi Akbari; Shohreh Jalaie

E-ISSN : 2148-9696

Crescent Journal of
Medical and Biological Sciences

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Apr 2021, Vol 8, Issue 2

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Original Article
The Preventive Role of Low-Level Laser Therapy in Cochlear Outer Hair Cell Damage Due to Noise Exposure in Guinea Pigs
Ahmad Zeinalzade¹, Ghassem Mohammadkhani², Mehdi Akbari³, Shohreh Jalaie⁴
¹School of Rehabilitation Sciences, Tehran University of Medical Sciences, Tehran, Iran ²Department of Audiology, School of Rehabilitation Sciences, Tehran University of Medical Sciences, Tehran, Iran ³Department of Audiology, School of Rehabilitation Sciences, Iran University of Medical Sciences, Tehran, Iran ⁴Department of Physiotherapy, School of Rehabilitation Sciences, Tehran University of Medical Sciences, Tehran, Iran
CJMB 2021; 8: 093-098 Viewed : 2129 times Downloaded : 1554 times. Keywords : Low-level laser, Caspase-3, Guinea-pigs, Apoptosis
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Abstract

Objectives: By considering the necessity prevention of hearing loss secondary to noise exposure, this research investigated the protective role of low-level laser (LLL) therapy in the prevention of cochlear outer hair cell (OHC) damage and death due to noise exposure in guinea-pigs. Materials and Methods: In this study, a total of 30 male albino guinea-pigs (290 ± 10 g) were used and randomly divided into three groups in order to evaluate hair cell counts and apoptosis of cochlear hair cells, including noise, control, and LLL therapy group each containing 10 pigs. The right outer ear canal of the LLL therapy group was exposed to165 mW/cm² LLL for five successive days for just half an hour per day. Then, the LLL therapy and noise groups were exposed to 3-6 kHz octave band noise at 120 dBSPL. Twenty-one days after noise exposure, the animals of all groups were killed for the count of their OHCs and immunohistochemistry for caspase-3 experiments. Results: The percentage of OHCs in the base and middle turns of the cochlea was significantly lower in the noise group compared to the control and low laser therapy groups (P < 0.05). The expression of caspase-3 significantly differed in the noise group in comparison to control and low laser groups regarding the experiment of immunohistochemistry (P < 0.05). Conclusions: The findings indicated that LLL was useful in the protection of the cochlear OHCs and could help in the prevention of hair-cell apoptosis.