Molecular Fingerprinting and Occurrence of bla<sub>KPC</sub> and bla<sub>OXA</sub>-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran

Gholamreza Goudarzi; Pegah Shakib; Khatereh Anbari; Shahram Shokri

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Original Article
Molecular Fingerprinting and Occurrence of bla_KPC and bla_OXA-48 in Klebsiella pneumoniae Isolated From Clinical Specimens, Khorramabad, Iran
Gholamreza Goudarzi¹, Pegah Shakib¹, Khatereh Anbari², Shahram Shokri³
¹Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran ²Department of Community Medicine, Lorestan University of Medical Sciences, Khorramabad, Irann. ³Department of Infectious Diseases, Lorestan University of Medical Sciences, Khorramabad, Iran
CJMB 2020; 7: 348-354 Viewed : 15809 times Downloaded : 2186 times. Keywords : Carbapenemase, Klebsiella pneumoniae, bla_OXA-48, bla_KPC
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Abstract

Objectives: Carbapenem-resistant Klebsiella pneumoniae (CR-Kp) is created by various mechanisms mainly including the production of Klebsiella pneumoniae carbapenemase (KPC) and other class A, B, and D beta-lactamases (e.g., OXA-48). The present study was undertaken to assess the occurrence of bla_KPC and bla_OXA-48 genes among K. pneumoniae isolates. Materials and Methods: In this cross-sectional study, 110 K. pneumoniae strains were collected from clinical samples from 2 teaching hospitals in Khorramabad, Iran. Susceptibility testing and modified-Hodge test (MHT) for K. pneumoniae isolates were performed according to Clinical and Laboratory Standards Institute procedures, followed by carrying out the detection of bla_KPC and bla_OXA-48 genes by polymerase chain reaction (PCR). Finally, genetic similarity among the tested strains tested was determined by the repetitive extragenic palindromic PCR (rep-PCR) method. Results: Up to 110 K. pneumoniae, the highest resistance and susceptibility rates pertained to ampicillin and ertapenem, respectively. In addition, 10 (9%) isolates were considered as carbapenemase-producing K. pneumoniae by the MHT. Although 13 (11.8%) isolates were bla_OXA-48-positive, none of them were harboring bla_KPC. Eventually, 13 and 44 clusters were distinguished in hospitals A and B by rep-PCR, respectively. Conclusions: The results of this study indicated that resistance to carbapenems among K. pneumoniae isolates were relatively common in our region. Moreover, the disseminated clones in our hospitals had mainly diverse origins.