Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM

Shiva Pour Vahdani; Taghi Khanzadeh; Fattaneh Karimi; Aylin Jahanban Esfahlan; Somayeh Ghavipanjeh; Amin Ghasemi; Milad Zadi Heydarabad; Hakim Azizi; Akbar Darbin; Arash Khorrami; Ako Azimi

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Apr 2019, Vol 6, Issue 2

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Original Article
Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM
Shiva Pour Vahdani¹, Taghi Khanzadeh², Fattaneh Karimi³, Aylin Jahanban Esfahlan⁴, Somayeh Ghavipanjeh⁵, Amin Ghasemi¹, Milad Zadi Heydarabad⁶, Hakim Azizi⁷, Akbar Darbin⁸, Arash Khorrami⁹, Ako Azimi⁹
¹Student Research Committee, Maragheh University of Medical Sciences, Maragheh, Iran ²Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ³Department of Community and Mental Health Nursing, School of Nursing and Midwifery, Maragheh University of Medical Sciences, Maragheh, Iran ⁴Department of Nursing, School of Nursing and Midwifery, Maragheh University of Medical Sciences, Maragheh, Iran ⁵Department of Psychiatric Nursing, Maragheh University of Medical Sciences, Maragheh, Iran ⁶Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran ⁷Department of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran ⁸Shahid Madani Hospital, Tabriz University of Medical Sciences, Tabriz, Iran ⁹Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
CJMB 2019; 6: 209-213 Viewed : 5056 times Downloaded : 3185 times. Keywords : Leukemia, Resveratrol, BCL-2 associated X, BCL-2 Gene, Apoptosis
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Abstract

Objectives: Acute lymphoblastic leukemia (ALL) is considered one of the common types of cancers in childhood with an incidence of up to 25%. In addition, drug resistance is a phenomenon which reduces the chances of overcoming cancer. Further, a phytoalexin combination called resveratrol can sensitize the leukemic cells to apoptotic cell death. Due to the importance of the above-mentioned issues, the present study aimed to evaluate the effect of resveratrol on BAX and BCL-2 expression levels and apoptosis induction. Materials and Methods: CCRF-CEM cultured cells were treated by resveratrol at doses of 15, 50, and 100 μM based on previous studies. Furthermore, RT Polymerase chain reaction (PCR) was conducted to assess the BAX and BCL-2 gene expression. Moreover, the amount of apoptosis induction was analyzed by annexin V staining method. Results: Based on the results, time and concentration were found to play a critical role in resveratrol-induced apoptosis. Additionally, BAX upregulation and BCL-2 downregulation excreted by resveratrol in CCRF-CEM cells resulted in predisposing these cells to apoptosis. Conclusions: In general, it was revealed that resveratrol could have a chemo-preventive activity by modifying the expression of BAX and BCL-2 genes. Finally, resveratrol was found to be a supplement drug in anti-leukemic therapy.