Downregulation of Autophagy-related Genes in Macrophages from Patients with Behcet's Disease

Mohammad Taghi Palizgir; Maryam Akhtari<sup>1; 2</sup>; Farhad Shahram; Shayan Mostafaei; Maassoomeh Akhlaghi; Soheila Sobhani; Mahdi Mahmoudi

E-ISSN : 2148-9696

Crescent Journal of
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Original Article
Downregulation of Autophagy-related Genes in Macrophages from Patients with Behcet's Disease
Mohammad Taghi Palizgir¹, Maryam Akhtari^1,2, Farhad Shahram¹, Shayan Mostafaei¹, Maassoomeh Akhlaghi¹, Soheila Sobhani¹, Mahdi Mahmoudi¹,
¹Rheumatology Research Center, Tehran University of Medical Sciences, Tehran, Iran ²Department of Cell & Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
CJMB 2018; 5: 014-020 Viewed : 4043 times Downloaded : 3041 times. Keywords : Behcet disease, Autophagy-related genes, Macrophage, Inflammation
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Abstract

Objective: Overwhelming inflammatory chemokines and cytokines characterize the immunological profile and inflammatory settings of Behcet disease (BD). The connection between autophagy-related genes (ATGs) and various perspectives of innate and adaptive immunobiology such as antigen presentation, immune tolerance, lymphocyte development and differentiation, cytokine signaling, and inflammation have been implicated. The aim of this study was to evaluate the mRNA expression profile of ATGs in macrophages of patients with BD. Materials and Methods: Whole blood samples were obtained from 10 BD patients and 10 healthy controls. Monocytes were isolated from the blood samples and then differentiated to macrophages using macrophage colony-stimulating factor (M-CSF). After total RNA extraction and cDNA synthesis, quantitative analysis of ATGs including ATG5, ATG7, ATG12, LC3b, mTOR, RAPTOR, and RICTOR was conducted by SYBR Green master mix and real-time polymerase chain reaction (PCR). Results: mRNA expression of all ATGs was downregulated in macrophages of BD patients compared with healthy controls. It is worth to note that the downregulation of ATG12 and LC3b mRNAs in macrophages of BD patients was statistically significant in comparison to that of healthy control group (P = 0.007 and 0.021, respectively). Conclusion: Considering the role of autophagy in initiation of immune responses and then clearance of dead cells as well as its participation in the development and differentiation of immune cells, downregulation of ATGs in macrophages of BD patients may be involved in uncontrolled immune response and overproduction of inflammatory cytokines.