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E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Apr 2024, Vol 11, Issue 2
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Original Article
Determination of the Protective Impact of Hypericum perforatum Extract Against Ovarian Ischemia and Reperfusion in Rats
Seyit Ali Bingöl1, Serdar Yiğit1, Ergin Taşkın2, Fatma Necmiye Kacı3, Muhammed Yayla4, Nurcan Kılıc Baygutalp5, Erdem Toktay1, Nilnur Eyerci6, Hasan Çılgın7
1Department of Histology and Embryology, Faculty of Medicine, Kafkas University, Kars, 36100, Türkiye
2Department of Medical Biochemistry, Faculty of Medicine, Agri Ibrahim Cecen University, Ağrı, 04100, Türkiye
3Department of Molecular Biology and Genetics, Faculty of Science, Erzurum Technical University, Erzurum, 25050, Türkiye
4Pharmacology Department, Faculty of Medicine, Kafkas University, Kars, 36100, Türkiye
5Department of Biochemistry, Faculty of Pharmacy, Ataturk University, Erzurum, 25240, Türkiye
6Department of Medical Biology, Faculty of Medicine, Kafkas University, Kars 36100, Türkiye
7Medical Park Hospital, Department of Obstetrics and Gynaecology Elazığ, Türkiye (Formerly Department of Obstetrics and Gynaecology, Faculty of Medicine, Kafkas University, Kars, 36100, Türkiye)

CJMB 2024; 11: 062-068
DOI: 10.34172/cjmb.2024.3720
Viewed : 988 times
Downloaded : 773 times.

Keywords : Ovary, Ischemia/reperfusion, Antioxidant, p53, PTEN
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Objectives: Ovarian torsion is an important gynaecological emergency because it causes ischemia in ovary. Our aim is to research the effects of Hypericum perforatum on p53, TNF-α, CAT, SOD, GSH, PTEN and is haPI3K/Akt/mTOR pathway in ovarian of rats with ischemia reperfusion (I/R) injury.

Materials and Methods: The study included 56 adult female rats which were allocated to 7 groups; control, ischemia, ischemia 300HP, ischemia 600HP, I/R, I/R 300HP and I/R 600HP. H. perforatum was applied to ischemia 300HP, ischemia 600HP, I/R 300HP and I/R 600HP groups at the dose of 300 mg/kg or 600 mg/kg by oral gavage. Collected ovarian tissues were examined by light microscopy, biochemical and real-time PCR techniques.

Results: In histological examination, the least degeneration was seen in 600HP group among other groups, apart from control group. TUNEL results showed that apoptosis level of other groups was lower than that of ischemia and I/R groups. CAT and GSH levels of I/R 600HP group increased. The highest level of PTEN and mTOR was found in the ischemia group. The Akt level was lower in I/R and I/R 300HP groups but its level was close to that of control and I/R 600HP groups. TNF-α and p53 mRNA expressions in I/R group were increased.

Conclusions: The result of the study showed that H. perforatum (600 mg/kg) had an effect on tissue degeneration, CAT and GSH levels, and also TNF-α and p53 mRNA expressions levels. We suggest that H. perforatum can be considered as an agent to protect against tissues damage during ischemia reperfusion.


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Articles by Ali Bingol S
Articles by Yiğit S
Articles by Taşkın E
Articles by Necmiye Kacı F
Articles by Yayla M
Articles by Toktay E
Articles by Eyerci N
Articles by Ocılgın H


Articles by Seyit Ali Bingöl
Articles by Serdar Yiğit
Articles by Ergin Taşkın
Articles by Muhammed Yayla
Articles by Erdem Toktay
Articles by Nilnur Eyerci
Articles by Hasan Çılgın

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