Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line

Parviz Faraji; Mostafa Araj-Khodaei; Amir Jafari; Maryam Ghaffari; Reza Mohammadinasab; Sanaz Hamedeyazdan; Miguel de la Guardia; Jafar Ezzati Nazhad Dolatabadi

doi:10.34172/cjmb.2022.31

E-ISSN : 2148-9696

Crescent Journal of
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Original Article
Anti-cancer Effects of Methotrexate in Combination With Melissa officinalis on HeLa Cancer Cell Line
Parviz Faraji^1,2, Mostafa Araj-Khodaei^1,3, Amir Jafari⁴, Maryam Ghaffari², Reza Mohammadinasab⁵, Sanaz Hamedeyazdan⁶, Miguel de la Guardia⁷, Jafar Ezzati Nazhad Dolatabadi⁸
¹Department of Persian Medicine, School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran ²Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran ³Neurosciences Research Center, Aging Research Institute, Tabriz University of Medical Sciences, Iran ⁴Pharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ⁵Department of History of Medicine, School of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran ⁶Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran ⁷Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain ⁸Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
CJMB 2022; 9: 189-194 DOI: 10.34172/cjmb.2022.31 Viewed : 1875 times Downloaded : 1781 times. Keywords : Methotrexate, Melissa officinalis, HeLa cancer cells, MTT assay, Flow cytometry
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Abstract

Objectives: One of the well-accepted beliefs about natural products, considering the advances of recently appearing new edges and features of herbal medicine, is paying more attention to cancer treatments. However, they have not been properly studied with reasonable/reliable clinical trials in human subjects in most cases. Therefore, seeking in vitro effects of herbs like Melissa officinalis (MO) in cancer therapy to identify the involved possible mechanism in conjugation with configurative/morphological aspects of treated cells seems quite necessary. In this study, we evaluated the co-treatment effect of anti-cancer drug methotrexate (MTX) and MO on HeLa cancer cells. Methods: MTT assay was applied to assess the quantitative cytotoxicity effect of both MTX and Mo. Apoptosis assay via flow cytometry was used to determine the amount of apoptotic and necrotic cells. To further investigate the anti-cancer effects, DAPI staining and DNA ladder assays are used qualitatively to detect changes in the nuclei of cells that are a sign of apoptosis occurring and morphological modifications of DNA. Results: MTX and MO mixture showed high cytotoxicity and apoptosis rate compared to untreated cells. Furthermore, the morphological changes of MTX and MO mixture were more evident than that of single MO, MTX, and control groups. Conclusions: These data regarding cell growth reduction and apoptosis induction in HeLa cancer cells showed that MTX and MO mixture can be an appropriate platform for cancer therapy.