Login Contact Us
E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Apr 2021, Vol 8, Issue 2
Advanced Search
Title
Authors
Keyword
Poll
How do you find the scientific quality of the published articles on our web site?
Original Article
The Preventive Role of Low-Level Laser Therapy in Cochlear Outer Hair Cell Damage Due to Noise Exposure in Guinea Pigs
Ahmad Zeinalzade1, Ghassem Mohammadkhani2, Mehdi Akbari3, Shohreh Jalaie4
1School of Rehabilitation Sciences, Tehran University of Medical Sciences, Tehran, Iran
2Department of Audiology, School of Rehabilitation Sciences, Tehran University of Medical Sciences, Tehran, Iran
3Department of Audiology, School of Rehabilitation Sciences, Iran University of Medical Sciences, Tehran, Iran
4Department of Physiotherapy, School of Rehabilitation Sciences, Tehran University of Medical Sciences, Tehran, Iran

CJMB 2021; 8: 093-098

Viewed : 642 times
Downloaded : 593 times.

Keywords : Low-level laser, Caspase-3, Guinea-pigs, Apoptosis
Full Text(PDF) | Related Articles
Abstract
Objectives: By considering the necessity prevention of hearing loss secondary to noise exposure, this research investigated the protective role of low-level laser (LLL) therapy in the prevention of cochlear outer hair cell (OHC) damage and death due to noise exposure in guinea-pigs.

Materials and Methods: In this study, a total of 30 male albino guinea-pigs (290 ± 10 g) were used and randomly divided into three groups in order to evaluate hair cell counts and apoptosis of cochlear hair cells, including noise, control, and LLL therapy group each containing 10 pigs. The right outer ear canal of the LLL therapy group was exposed to165 mW/cm2 LLL for five successive days for just half an hour per day. Then, the LLL therapy and noise groups were exposed to 3-6 kHz octave band noise at 120 dBSPL. Twenty-one days after noise exposure, the animals of all groups were killed for the count of their OHCs and immunohistochemistry for caspase-3 experiments.

Results: The percentage of OHCs in the base and middle turns of the cochlea was significantly lower in the noise group compared to the control and low laser therapy groups (P < 0.05). The expression of caspase-3 significantly differed in the noise group in comparison to control and low laser groups regarding the experiment of immunohistochemistry (P < 0.05).

Conclusions: The findings indicated that LLL was useful in the protection of the cochlear OHCs and could help in the prevention of hair-cell apoptosis.

 

Cite By, Google Scholar

Google Scholar

Articles by Zeinalzade A
Articles by Mohammadkhani G
Articles by Akbari M
Articles by Jalaie S

PubMed

Articles by Ahmad Zeinalzade
Articles by Mehdi Akbari
Articles by Shohreh Jalaie

Submit Paper
Online Submission System
CJMB ENDNOTE ® Style Tutorials Publication Charge Women's Reproductive Health Research Center About Journal
Publication Information
Publisher
Aras Part Medical International Press Editor-in-Chief
Arash Khaki
Deputy Editor
Zafer Akan
Published Article Statistics