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E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Jul 2020, Vol 7, Issue 3
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Original Article
Effect of Selenium and Electrical Stimulation on the Expression of Cation Channel Spermia 1 and 2 Genes in Spinal Cord Injury Rat Model
Soheila Bani1, Iraj Lotfinia2, Parviz Shahabi2, Abolfazl Barzegari3, Jalal Abdolalizadeh4, Amir Vahedi5, Meysam Ghorbani2
1Pediatric Health Research Center, Nursing and Midwifery Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
2Neurosciences Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Research Center for Pharmaceutical Nanotechnology, Tabriz University of Medical Sciences, Tabriz, Iran
4Drug Applied Research Center, Paramedical Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
5Department of Pathology, Tabriz University of Medical Sciences, Tabriz, Iran

CJMB 2020; 7: 382-388

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Keywords : Selenium, Electrical stimulation, CatSper 1 and 2 genes
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Abstract
Objectives: Cation Channel Spermia (CatSper) 1-4 are considered as a unique family of "sperm cation channels" and play an important role in the motility of the sperm and male fertility. The aim of this study was to compare and evaluate the effects of selenium (Se) and electrical stimulation (Es) on the expression of CatSper 1 and 2 genes in the spinal cord injury (SCI) rat model.

Materials and Methods: The present experimental study was performed on 50 male Wistar rats. The animals were randomly divided into 5 groups of 10 which experienced SCI using an SCI impactor device. Forty-eight hours after injury, the Se group received Se for 6 consecutive days for 6 weeks, and Es was performed for 1 hour per day for 6 consecutive days during 6 weeks in the Es group. Finally, the real-time polymerase chain reaction (PCR) was performed for the expression of CatSper 1 and 2 genes, followed by using one-way ANOVA for the analysis of gene expression data. The results were considered statistically significant when P < 0.05.

Results: Se treatment showed an increase in the relative expression of CatSper 1 and 2 genes compared to the control group, but this increase was higher for CatSper 2. Eventually, Es did not affect the relative intensity of CatSper 1 and 2 gene expression after SCI.

Conclusions: Se, more than Es, could increase the relative intensity of CatSper1 and 2 expressions, and this increase was higher in CatSper2.

 

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Articles by Bani S
Articles by Lotfinia I
Articles by Shahabi P
Articles by Barzegari A
Articles by Abdolalizadeh J
Articles by Vahedi A
Articles by Ghorbani M

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Articles by Soheila Bani
Articles by Iraj Lotfinia
Articles by Parviz Shahabi
Articles by Abolfazl Barzegari
Articles by Amir Vahedi
Articles by Meysam Ghorbani

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