|Simultaneity Metformin and Sitagliptin Effect on Proteins Content Involved in Insulin Resistance in Human Adipose Tissue of Type 2 Diabetes: A Single-Arm Clinical Trial|
|Reza Didehdar1, Yousof Naghiaee2, Javad Mohiti-Ardekani1, Naeimeh Heyranizadeh3, Masaoud Rahmanian4|
|1Department of Biochemistry and Molecular Biology, Faculty of Medicine, International Campus Of Shahid Saduoghi University of Medical Sciences, Yazd, Iran
2Department of Biochemistry and Molecular Biology, Faculty of Medicine, Shahid Saduoghi University of Medical Sciences, Yazd, Iran
3Department of General Surgery, Faculty of Medicine, Shahid Saduoghi University of Medical Sciences, Yazd, Iran
4Center Of Research-Therapy Diabetes, Shahid Saduoghi University of Medical Sciences, Yazd, Iran
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Keywords : Insulin Resistance, Metformin, Sitagliptin, Type2 Diabetes
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Objectives: A cause of persistent insulin resistance and type II diabetes is obesity, which finally leads to a wide range of metabolic alterations, the most important of which is insulin resistance. In insulin resistance, the mechanism of insulin signal to intra-cells, chiefly in adipose tissue cells happens, which by identifying the proteins this pathway, can be suitable targets for therapeutic approaches. In this study, we wanted to know whether insulin resistance is simultaneously broken down by these two drugs, and if it is, what changes occurs in intended proteins levels in insulin signaling pathway.
Materials and Methods: This article is a single-arm clinical trial. We studied 6 persons of type 2 diabetic patients that were 3 months treatment with simultaneity metformin and sitagliptin, that 4 persons from them returned after treatment and 8 persons as healthy case (control group), To determine the content of proteins involved in insulin resistance GLUT4, Akt, PI3K, IRS-1 and mTOR in adipose tissue of diabetic patients with use of SDSPAGE and western blot analyses.
Results: Following simultaneity metformin and sitagliptin treatment, increases in glucose disposal and decreases in serum glucose levels (p<0.05) and decreased insulin resistance (p<0.05), and altered in serum insulin levels. On the other, increase the content of proteins involved in insulin resistance increase of GLUT4, Akt, IRS-1, PI3K and mTOR in adipose tissue of diabetic patients were observed (p<0.05).
Conclusion: Simultaneity metformin and sitagliptin treatment statistic significantly improved proteins involved in insulin resistance GLUT4, Akt, IRS-1, PI3K and mTOR in type2 diabetic adipose tissue.
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