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E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Jan 2019, Vol 6, Issue 1
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Original Article
Validation of Total RNA Spin Column and Non-column Extraction Methods for microRNA Expression Analyses in FFPE Liver Samples
Zahra Asefy1, Naser Samadi1, AlirezaAbhari1, Behrooz Shokuhi1, Saeed Nazare1,2, Sirus Hoseinnejhad3, Mohammad Noori1
1Department of Clinical biochemistry, Tabriz university of medical sciences, Tabriz-Iran
2Digestive and Liver Research Center, Tabriz university of medical sciences, Tabriz-Iran
3 Department of Biochemistry, Baku State University, Baku, Azerbaijan

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Keywords : Liver,Spin column extraction, miRNA,FFPE
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Objectives: MicroRNAs are small (19–23nt), non coding RNAs that adjust gene expression post transcriptionally. miRNAs profile Modification has been revealed in various abnormalities , such as cancer. Formalin-fixed, paraffin-embedded (FFPE) materials archives are valued to search human diseases.

Material and Methods:For comparison on archived specimens between these methods we study a qualitative and quantitative assessment of the two extracting methods. In each method, we quantify total RNA and gene expression.

Results: RNA extracted from FFPE tissues and RTPCR of hsa-miR-623, hsa-miR-515-5pin miRNeasy FFPE(P -value <0.047) significantly higher than that in RNAX Plus(P -value <0.084).

Conclusion: Spin column method miRNA expression profile had better expression that from non column method . These results also show FFPE tissues validity as appropriate resources for miRNA analyses.


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Articles by Asefy Z
Articles by Samadi N
Articles by Alirezaabhari A
Articles by Shokuhi B
Articles by Nazare S
Articles by Hoseinnejhad S
Articles by Noori M


Articles by Zahra Asefy
Articles by Naser Samadi
Articles by AlirezaAbhari
Articles by Behrooz Shokuhi
Articles by Saeed Nazare
Articles by Sirus Hoseinnejhad
Articles by Mohammad Noori

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Aras Part Medical International Press Editors in Chief
Arash Khaki
Zafer Akan Deputy Editor
Javadi, Leila
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