|Validation of Total RNA Spin Column and Non-column Extraction Methods for Micro RNA Expression Analyses in FFPE Liver Samples|
|Zahra Asefy1,2,3, Alireza Abhari2, Behrooz Shokuhi1, Zeinab Latifi2, Saeed Nazari Soltan Ahmad1,2, Sirus Hoseinnejhad4, Naser Samadi2, Mohammad Nouri1,2|
|1Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Department of Biochemistry and Clinical Laboratories, Tabriz University of Medical Sciences, Tabriz, Iran
3Student’s Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
4Department of Biochemistry, Baku State University, Baku, Azerbaijan
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Keywords : Liver,Spin column extraction, miRNA,FFPE
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Objectives: MicroRNAs are small (19–23nt), non coding RNAs that adjust gene expression post transcriptionally. miRNAs profile Modification has been revealed in various abnormalities , such as cancer. Formalin-fixed, paraffin-embedded (FFPE) materials archives are valued to search human diseases.
Material and Methods:For comparison on archived specimens between these methods we study a qualitative and quantitative assessment of the two extracting methods. In each method, we quantify total RNA and gene expression.
Results: RNA extracted from FFPE tissues and RTPCR of hsa-miR-623, hsa-miR-515-5pin miRNeasy FFPE(P -value <0.047) significantly higher than that in RNAX Plus(P -value <0.084).
Conclusion: Spin column method miRNA expression profile had better expression that from non column method . These results also show FFPE tissues validity as appropriate resources for miRNA analyses.
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