|Validation of Total RNA Spin Column and Non-column Extraction Methods for microRNA Expression Analyses in FFPE Liver Samples|
|Zahra Asefy1, Naser Samadi1, AlirezaAbhari1, Behrooz Shokuhi1, Saeed Nazare1,2, Sirus Hoseinnejhad3, Mohammad Noori1|
|1Department of Clinical biochemistry, Tabriz university of medical sciences, Tabriz-Iran
2Digestive and Liver Research Center, Tabriz university of medical sciences, Tabriz-Iran
3 Department of Biochemistry, Baku State University, Baku, Azerbaijan
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Keywords : Liver,Spin column extraction, miRNA,FFPE
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Objectives: MicroRNAs are small (19–23nt), non coding RNAs that adjust gene expression post transcriptionally. miRNAs profile Modification has been revealed in various abnormalities , such as cancer. Formalin-fixed, paraffin-embedded (FFPE) materials archives are valued to search human diseases.
Material and Methods:For comparison on archived specimens between these methods we study a qualitative and quantitative assessment of the two extracting methods. In each method, we quantify total RNA and gene expression.
Results: RNA extracted from FFPE tissues and RTPCR of hsa-miR-623, hsa-miR-515-5pin miRNeasy FFPE(P -value <0.047) significantly higher than that in RNAX Plus(P -value <0.084).
Conclusion: Spin column method miRNA expression profile had better expression that from non column method . These results also show FFPE tissues validity as appropriate resources for miRNA analyses.
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