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E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Jul 2019, Vol 6, Issue 3
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Original Article
Validation of Total RNA Spin Column and Non-column Extraction Methods for Micro RNA Expression Analyses in FFPE Liver Samples
Zahra Asefy1,2,3, Alireza Abhari2, Behrooz Shokuhi1, Zeinab Latifi2, Saeed Nazari Soltan‎ Ahmad1,2, Sirus Hoseinnejhad4, Naser Samadi2, Mohammad Nouri1,2
1Liver and Gastrointestinal Diseases Research Center‎, Tabriz University of Medical Sciences, Tabriz, Iran
2Department of Biochemistry and Clinical Laboratories, Tabriz University of Medical Sciences, Tabriz, Iran
3Student’s Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
4Department of Biochemistry, Baku State University, Baku, Azerbaijan


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Keywords : Liver,Spin column extraction, miRNA,FFPE
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Abstract
Objectives: MicroRNAs are small (19–23nt), non coding RNAs that adjust gene expression post transcriptionally. miRNAs profile Modification has been revealed in various abnormalities , such as cancer. Formalin-fixed, paraffin-embedded (FFPE) materials archives are valued to search human diseases.

Material and Methods:For comparison on archived specimens between these methods we study a qualitative and quantitative assessment of the two extracting methods. In each method, we quantify total RNA and gene expression.

Results: RNA extracted from FFPE tissues and RTPCR of hsa-miR-623, hsa-miR-515-5pin miRNeasy FFPE(P -value <0.047) significantly higher than that in RNAX Plus(P -value <0.084).

Conclusion: Spin column method miRNA expression profile had better expression that from non column method . These results also show FFPE tissues validity as appropriate resources for miRNA analyses.

 

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Articles by Asefy Z
Articles by Abhari A
Articles by Shokuhi B
Articles by Latifi Z
Articles by Hoseinnejhad S
Articles by Samadi N
Articles by Nouri M

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Articles by Zahra Asefy
Articles by Alireza Abhari
Articles by Behrooz Shokuhi
Articles by Zeinab Latifi
Articles by Sirus Hoseinnejhad
Articles by Naser Samadi
Articles by Mohammad Nouri

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Aras Part Medical International Press Editors in Chief
Arash Khaki
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Zafer Akan Deputy Editor
Javadi, Leila
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