|Effect of Vitamin E and Sodium Selenite on the Expression of Bax and Bcl2 Genes and Renal Histopathology in the Electromagnetic Field Exposed Mice|
|Abbas Majdi1, Mohammad Eyvazi1, Hamid Tayefi1, Ali Abedelahi1, Ramin Salimnegad|
|1Department of Anatomical Science, Faculty of Medicine, Tabriz University of Medical Sciences,Tabriz ,Iran|
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Keywords : EMF, kidney, vitamin E, sodium selenite, Bax, Bcl2
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Objectives : Electromagnetic field (EMF) causes damage to the kidney through the increase of oxidative stress. The use of antioxidants can reduce the effects induced by EMF. The present study investigates the effects of vitamin E and sodium selenite on the Bax and Bcl2 genes expression renal histopathology in the EMF exposed mice exposed.
Materials and Methods: In this study, 48 mice with the approximate weight of 24-28 g were used.The mice were randomly divided into six groups (n=8): 1) control 2); EMF; 3) EMF + vitamin E; 4) EMF + Sodium selenite; 5) EMF + vitamin E + Sodium selenite; and 6) vitamin E + sodium selenite. Rats were exposed to the EMFfor 4 hours a day for 2 months. Finally, after 2 months, the mice sacrified by cervical dislocation and the right kidney was taken for the evaluation of the histopathological changes and the left kidney was used to determine the expression of Bax and Bcl-2 genes by Real Time PCR.
Results: Statistical analyses showed that EMF significantly decreased the kidney corpuscle diameter and bcl2 gene expression, as well as the increase of urinary space, basal membrane thickness, and the increase of Bax gene expression compared to the control group (p<0.05). The treatment of mice by vitamin E and sodium selenite showed that administration of these antioxidants during exposure to EMF significantly decreased the effects of EMF on these parameters (p<0.05).
Conclusion: The results of this study showed that vitamin E and sodium selenite significantly inhibited the destructive effects of EMF on the expression of Bax and bcl2 genes and prevented histopathological changes of mice kidney.
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