|Mummy Material Enhances Chondrocyte Proliferation and The Expression of Extracellular Matrix Components in Monolayer and 3D Culture Conditions|
|Azadeh Montaseri1, Sina Parsay2, Maryam Hassan Famian3, Fariba Rezaei1, Hajar Shafaei1, Mohammad Reza Aliparasti4, Jafar Soleimani Rad1|
|1Stem Cells Research Center, Anatomical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
2Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran
3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
4Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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Keywords : Extracellular matrix, Chondrocytes, Proliferation, Gene expression, Osteoarthritis
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Objectives: Mummy has been traditionally applied by Persian indigenous people for alleviation of different diseases such as bone fractures, wounds and joint inflammation. The aim of this study is to investigate the probable effects of Mummy on chondrocyte proliferation and expression of cartilage specific gene.
Materials and Methods: Chondrocytes at the second passage were plated at density of 1x106 in monolayer or seeded onto PCL scaffolds. Cells in both conditions were divided into control (received medium) or treated with Mummy at concentrations of 500 or 1000 µg/ml for 72 hours. The gene expression profile of collagen II, aggrecan, cartilage oligomeric matrix protein (COMP) and sox-9 was evaluated using real-time RT-PCR. The ability of Mummy to enhance the proliferation of chondrocytes was further evaluated using doubling time.
Results: Treatment of chondrocytes with 1000µg/ml of Mummy resulted in a significant increase in expression of Sox-9, COMP and Aggrecan genes in both monolayer and 3D culture conditions. Compared to the monolayer cultivated cells, expression of genes in seeded chondrocytes on PCL scaffolds was significantly higher. The highest proliferation rate of chondrocytes was found at a concentration of 1000µg/ml.
Conclusion: The preliminary findings of this study revealed that Mummy can be a promoting factor for chondrocyte proliferation and ECM synthesis. The use of Mummy at 1000µg/ml may be a suitable dose for cartilage tissue engineering.
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