|Adipose Derived Stem Cells Conditioned Medium Can Enhance the Expression Level of Cartilage Specific Genes by Chondrocytes|
|Seyyedeh Mahdiye Hatami1, Sina Parsay2, Maryam Hassan Famian1, Masoumeh Kazemi3, Azadeh Montaseri3|
|1Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran
2Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
3Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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Keywords : Stem cells, Osteoarthritis, supernatant
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Objectives: Osteoarthritis (OA) is the most common multifactorial disease that deteriorates articular cartilage in elderly. Recently, mesenchymal stem cells are introduced as candidates for OA treatment but some researchers showed that implantation of stem cells into the lesion site of patients can lead to some side effects, so many scientists switches their field of research to the application of stem cells supernatant. The purpose this study is to evaluate whether supernatant of adipose-derived stem cells can result in expression of cartilage specific gene in chondrocyte.
Materials and Methods: Mesenchymal stem cells were isolated from adipose tissue and their supernatant was collected and added to chondrocytes. After 3 days, chondrocytes were prepared for expression of Collagen II, Sox9, COMP and Aggrecan genes using read the RT-PCR.
Results: Data analysis showed that in the presence of adipose-derived stem cells conditioned medium (ASC-CM) the gene expression level of Collagen II, Sox9, and Aggrecan did not change but that of the COMP significantly increased after 3 days.
Conclusion: Obtained results may be related to the unknown factors secreted by stem cells and more investigations are necessary for understanding the exact mechanisms underlying these findings.
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