|Pathogenesis Traits and Antimicrobial Resistance Pattern in Escherichia coli Isolates Recovered From Sewage|
|Behnaz Naraghi1, Mojtaba Afsharnia1, Jalal Mardaneh2, Mojtaba Kianmehr3, Hamed Biglari1, Javad Bazeli4, Mojtaba Anvarinejad5|
|1Department of Environmental Health Engineering, School of Public Health, Social Development & Health Promotion Research Center, Gonabad University of Medical Sciences, Gonabad, Iran
2Department of Microbiology, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
3Department of Medical Physics, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
4Nursing Department, Nursing & Midwifery, Gonabad University of Medical Sciences, Gonabad, Iran
5Professor Alborzi Clinical Microbiology Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
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Keywords : Escherichia coli, Sewage, Virulence
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Objectives: Escherichia coli is one of the most potential agents of community and hospital-acquired infections, and can readily acquire non-susceptibility to drugs used by animals and humans. E.coli, which has been broadly applied as indicator of a fecal contamination in aquatic environments, is routinely conducted non-virulence; nevertheless, some isolates can be virulence. The main objectives of this work were to survey antibiotic non-susceptibility, and charecterization of virulence factors and antibiotic resistance genes including traT, fimH, blaCTX, and tetA among the Escherichia coli isolates recovered from sewage in Gonabad, northeast of Iran.
Materials and Methods: In this cross-sectional work, from May 2016 to April 2017, a total of 99 non-duplicate strains of Escherichia coli were removed from three type sewage including poultry sewage(33 isolate), urban sewage(33 isolate), and livestock slaughter house sewage(33 isolate) in Gonabad, northeast of Iran. Antimicrobial susceptibility test and extended-spectrum-beta-lactamase(ESBL) production was done based on CLSI guidelines. The PCR technique was done for identification of 2 virulence genes(traT, and fimH) and 2 antibiotic resistance genes(blaCTX and tetA).
Results: Meropenem was the most effective drug against strains. The analysis of multiple drug non-susceptibility profiles in strains showed 39(39.3%) strains are TR-NAR. Three(3.03%) strains were resistant to colistin. Totally, twenty-one(21.2%) strains were ESBL-positive and 15(71.42%) ESBL+ isolates carried blaCTX gene, whereas respectively, 87.9% and 100% of the strains in livestock slaughter house sewage and urban sewage carried tetA gene. 85.7% ESBL+ isolates carried fimH gene. Overally, 19.8% ESBL positive strains carried fimH and traT virulence genes(fimH+-traT+ genotype).
Conclusion: This study has shown that wastewater and surface waters are a significant source of E. coli carrying ESBL-genes, high level of antibiotic non-susceptibility, and pathogenesis traits. Colistin non-susceptible strains identified in our work are quite worrying.
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