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E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Apr 2019, Vol 6, Issue 2
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Original Article
Genotyping, Pan Drug-Resistance, Extensively Drug-Resistant, and Multi Drug-Resistance Detection of Pseudomonas AeruginosaIsolated from Patients in the West of Iran
Samaneh Rouhi1,2, Rashid Ramazanzadeh2,3, Bijan Nouri4
1Student Research Committee, Kurdistan University of Medical Sciences, Sanandaj, Iran
2Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
3Department of Microbiology , Kurdistan University of Medical Sciences, Sanandaj, Iran
4Social Determinants of Health Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran

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Keywords : Genotyping, Pan Drug-Resistance, Extensively Drug-Resistant, Multi Drug-Resistance, Pseudomonas aeruginosa
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Objectives:Resistance to multiple antibiotic classes are calledmultidrug resistance (MDR), extremely drug resistance (XDR), and pandrug resistance (PDR). Genotyping survey of resistant bacteria is a prominent factor in epidemiological surveying. The aim of this study was epidemiological survey and XDR, MDR, and PDR detection of P. aeruginosa isolated from clinical specimens in Kurdistan province, Iran.

Materials and Methods:In a period from December 2015 to August 2017, a total of 134 strains of P. aeruginosa were detected by PCR and they were tested for antibiotic resistance, MDR, XDR, and PDR. Related specimens of patients with nosocomial infections were subjected to BOX-PCR analysis. Data analyses were performed using Stata software,V. 12 with frequency determination, Fisher’s exact test, and Logistic regression (p≤0.05). BOX-PCR analysis was done with GelJsoftware V.1.3.

Results:Thelowest and highest resistance rates and the highest and lowest susceptibility ratesin 134 isolates of P. aeruginosawere related to cefpodoxime (93.28%), imipenem(27.61%), colistin (69.40%), and cefpodoxime (6.71%), respectively. MDR and XDR isolates were observed in 97.76% and 15.67% ofP. aeruginosa isolates, respectively. PDR was not detected in any of the isolates. BOX-PCR showed fourmain clusters with 56 unique patterns in nosocomial infection isolates.A significant relationship was observedbetween MDR and XDR isolates and nosocomial infection (p≤0.05); and anotherbetween ward, type ofspecimens, and hospitals with nosocomial infection(p≤0.05). 

Conclusions:There was a high frequency of antibiotic resistance in this study. No genetic correlation was observed between P. aeruginosa strains. Proper selection of the most effective antibiotics anddevising an efficient way forcontrolling antibiotic resistance  should be taken into consideration.


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