|Evaluation of Cartilage Specific Genes Expression In Co-culture of Chondrocytes With Two Different Mesenchymal Stem Cells: Adipose and Wharton's Jelly Derived|
|Negin Farzamikia1, Maryam Zeynali2, Maryam Hassan Famian3, Sina Parsay4, Masoumeh Kazemi5, Azadeh Montaseri5|
|1Department of Biology, East Azerbaijan Science and Research Branch, Islamic Azad University, Tabriz, Iran
2Department of Biology, Basic Sciences Faculty, Islamic Azad University, Tabriz, Iran
3Department of molecular biology, Ahar Branch, Islamic Azad University, Ahar, Iran
4Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
5Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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Keywords : Adipose tissue, Cartilage ,Stem cells, Osteoarthritis, Wharton"s jelly
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Objectives : Articular cartilage has a limited potential for self-repairing due to scarce number of chondrocytes and also avascularity. Regarding to the low proliferative potential of chondrocyte in in vitro condition, nowadays mesenchymal stem cells are introduced for OA treatment. The purpose of this study was to evaluate whether co-culture of chondrocytes with two different sources of mesenchymal stem cells, adipose and Wharton's jelly derived can promote the expression of cartilage specific genes by chondrocytes.
Materials and Methods: Mesenchymal stem cells were isolated from both adipose tissue and Wharton’s jelly. Obtained cells were co-cultured with chondrocytes or cultivated alone as control for 3 days. After this time, using Real Time RT-PCR, cartilage specific genes including Collagen II, Sox9, cartilage oligomeric matrix protein (COMP) and Aggrecan were evaluated.
Results: The expression of genes in chondrocytes alone was significantly higher compared to the co-culture of chondrocytes with both stem cell types. Furthermore these data revealed that co-culture of WJSCs with chondrocyte resulted in higher expression of genes compared to WJSCs alone but that of the ASCs did not change significantly.
Conclusion: Co-cultivation of chondrocytes with ASCs or WJSCs did not result in up-regulation of cartilage specific genes compared to chondrocytes. Compared to the ASCs, stem cells derived from Wharton's jelly expressed higher levels of above mentioned genes.
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