Login Contact Us
E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Jan 2019, Vol 6, Issue 1
Advanced Search
Title
Authors
Keyword
Poll
How do you find the scientific quality of the published articles on our web site?
Original Article
Evaluating the Cartilage-Specific Genes Expression in Co-culture of Chondrocytes With 2 Different Mesenchymal Stem Cells: Adipose and Wharton"s Jelly Derived
Negin Farzamikia1, Maryam Zeynali2, Maryam Hassan Famian3, Sina Parsay4, Masoumeh Kazemi5, Azadeh Montaseri5
1Department of Biology, East Azerbaijan Science and Research Branch, Islamic Azad University, Tabriz, Iran
2Department of Biology, Basic Sciences Faculty, Islamic Azad University, Tabriz, Iran
3Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran
4Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
5Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

CJMB 2019; 6: 066-071

Viewed : 530 times
Downloaded : 296 times.

Keywords : Adipose tissue, Cartilage, Stem cells, Osteoarthritis, Wharton"s jelly
Full Text(PDF) | Related Articles
Abstract
Objectives: Articular cartilage has a limited potential for self-repairing due to the scarce number of chondrocytes and avascularity. As regards the low proliferative potential of chondrocyte under in vitro conditions, nowadays mesenchymal stem cells are introduced for osteoarthritis (OA) treatment. Accordingly, the present study aimed to evaluate whether co-culture of chondrocytes with 2 different sources of mesenchymal stem cells, namely, adipose and Wharton"s jelly derived can promote the expression of cartilage specific genes by chondrocytes.

Materials and Methods: Mesenchymal stem cells were isolated from both adipose tissue and Wharton"s jelly. The obtained cells were co-cultured with chondrocytes or cultivated alone as control for 3 days. Then, cartilage specific genes including collagen II, Sox9, cartilage oligomeric matrix protein (COMP), and aggrecan were evaluated using the real time Reverse transcription polymerase chain reaction (RT-PCR).

Results: The expression of genes in chondrocytes alone was significantly higher compared to the co-culture of chondrocytes with both stem cell types. In addition, the results revealed that co-culture of Wharton jelly derived stem cells (WJSCs) with chondrocyte led to higher expression of genes compared to WJSCs alone. However, the co-culture of the adipose-derived stem cells (ASCs) demonstrated no significant change.

Conclusions: Generally, no up-regulation of cartilage specific genes was observed by co-cultivation of chondrocytes with ASCs or WJSCs compared to chondrocytes. Further, stem cells derived from Wharton"s jelly expressed higher levels of the above mentioned genes compared to those of the ASCs.

 

Cite By, Google Scholar

Google Scholar

Articles by Farzamikia N
Articles by Zeynali M
Articles by Hassan Famian M
Articles by Parsay S
Articles by Kazemi M
Articles by Montaseri A

PubMed

Articles by Negin Farzamikia
Articles by Maryam Zeynali
Articles by Sina Parsay
Articles by Masoumeh Kazemi
Articles by Azadeh Montaseri

Submit Paper
Online Submission System
CJMB ENDNOTE ® Style Tutorials Publication Charge Women's Reproductive Health Research Center About Journal
Publication Information
Publisher
Aras Part Medical International Press Editors in Chief
Arash Khaki
Editor
Zafer Akan Deputy Editor
Javadi, Leila
Published Article Statistics