|Investigation of Autophagy-related Genes Expression in the Parkinson's disease Rat Model|
|Zoherh Golmohammadi1, Ali Noori-Zadeh2, Farzad Rajaei3, Leila Darabi3, Hatef Ghasemi Hamidabadi4, Hojjat-Allah Abbaszadeh5, Salar Bakhtiyari6, Mohammad Amin Abdollahifar7, Shahram Darabi3|
|1Faculty of medicine, Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran
2Department of Clinical Biochemistry, Faculty of Paramedicine, Ilam University of Medical Sciences, Ilam, Iran
3Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
4Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
5Hearing Disorders Research Center, Loghman Hakim Medical Center, Shahid Beheshti University of Medical Sciences.Department of Biology and AnatomicalSciences, School of Medicine, Shahid BeheshtiUniversity of Medical Sciences, Tehran, Iran
6Department of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
7Department of Anatomical Sciences and Biology, Medical School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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Keywords : 6-Hydroxydopamine, ATG 101, Autophagy, Oxidative stress, Parkinson's Disease
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Objectives: Parkinson's disease (PD) is characterized by protein aggregations in the cytoplasm of the dopaminergic neurons due to cellular stresses. In response to these stresses, autophagy is a conservative mechanism and dysregulation of it results in protein aggregation.Despite the proper acceptance of the prominent role of autophagy in PD, its associated-gene expression dysregulation involved in the autophagosome formation has remained largely unknown. In this study, the autophagy-related gene expressions in the PD rat model investigated.
Material and Methods: Male Wistar rats divided into control, sham and PD model experimental groups. By injection of 6-hydroxydopamine (6-OHDA) into the striatum, the rat model of PD was induced. The apomorphine-induced rotation test was done one week before (baseline) and four weeks after surgery and also Nissl staining was performed for the brain sections. Then, rat substantia nigra pars compacta extracted and RT-PCR was performed to detect expression of FOXO3A and the autophagy-related genes (ATG). Furthermore, using Western blotting, we investigated the protein levels of ATG101.
Results: Apomorphine-induced rotation test indicated significant contralateral rotations in the rat model group. Using RT-PCR, in the induction group, ATG101 did not express and ATG13, ATG14L, and VPS34 genes were downregulated in comparison with control groups. Furthermore, Western blotting showed that ATG101 protein does not express in the model group.
Conclusions: The results showed that deregulation of ATG101 expression, as a factor involved in the initial stages of the autophagy, occurs in in the rat model of PD.
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