|Epidemiology and Identification of Trichomonas vaginalis Genotypes in Women in Southeast of Iran by Actin-gene PCR-RFLP Method|
|Fateme Shahraki1, Bahman Fouladi2, Alireza Salimi-Khorashad3, Nahid Sepehri-Rad3, Mansour Dabirzadeh 1|
|1Department of Parasitology, Faculty of Medical Science. Zabol University of Medical Sciences, Zabol, Iran
2Department of Parasitology and Mycology, Faculty of Medical Science, Zabol university of Medical Sciences, Zabol, Iran
3Infectious Diseases and Tropical Medicine Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
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Keywords : Epidemiology, Trichomonas vaginalis, Actin gene, Zahedan, Zabol
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Objectives: Trichomoniasis is one of the most common sexually transmitted diseases in the world, which is caused by Trichomonas vaginitis and is the most commonly reported sexually transmitted disease after the viral infections and around 180 million people around the world experience this parasite every year. The infected people of this parasite exhibit a wide range of symptoms. As we didn’t have any information about the genetic variation, prevalence and related factors that affect the disease, this study was done.
Materials and Methods: 500 women patients who were referred to hospitals of Imam Khomeini hospitals in Zabol and Ali Ibn Abi Talib (AS) in Zahedan, Twenty-five positive clinical samples from vaginal discharge and urine were isolated by culture during 1959-1969. First DNA extraction was performed and then all samples were subjected to PCR using the nested-PCR method. Six different genotypes of actin gene have been identified by PCR-RFLP in Trichomonas vaginalis in Zahedan and Zabol. All of the PCR products were subjected to HindII and RsaI and MesI enzymes. All participants completed a questionnaire recommended by gynecologists and midwifery experts.
Results: As a result, genotypes, including H, G, E, I, N were obtained in this study. The dominant genotype of Trichomonas vaginalis in Zahedan and Zabol is genotype E. There was a significant difference between the type of clinical symptoms and the level of infection (p=0.0001).
Conclusion: Control of disease as a health problem must be conducted based epidemiological and genetic method. Control of the disease is related to education and drug resistance or sensitivity related to genetic variation and epidemiological factors.
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