Identification of Staphylococcus aureus in Synovial Fluid of Patients Suspected to Arthritis Through PCR in Urmia City

Afshin Taravati; Afshin Zahedi; Hiro Allipashaie; Mehdi Dilmagani

E-ISSN : 2148-9696

Crescent Journal of
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Original Article
Identification of Staphylococcus aureus in Synovial Fluid of Patients Suspected to Arthritis Through PCR in Urmia City
Afshin Taravati¹, Afshin Zahedi¹, Hiro Allipashaie², Mehdi Dilmagani³
¹Department of Veterinary Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran ²Department of Biotechnology, Urmia Branch, Islamic Azad University, Urmia, Iran ³Department of Cellular and Molecular Biology West Azerbaijan Veterinary Laboratory (WAVL), Urmia, Iran
CJMB 2016; 3: 128-131 Viewed : 2663 times Downloaded : 3108 times. Keywords : Synovial fluid, Arthritis, Staphylococcus aureus, PCR
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Abstract

Objective: Synovial fluid is composed of plasma ultrafiltration and hyaluronic acid secretion by synovial cells. Synovial fluid plays a role as softener and feeding cartilages without vessels. Infectious arthritis is one of the commonest arthritis and if the disease did not cure in the first days it would injure cartilages irreversibly. The goal of this study was identification of Staphylococcus aureus in synovial fluid of patients suspected to arthritis through PCR in Urmia city. Materials and Methods: In this research synovial fluid contamination with Staphylococcus aureus and biochemical parameters such as the amount of glucose, protein and the number of white blood cells were studied. Amplification of nuc gene with the length of 279 bp using PCR method was applied to confirm Staphylococcus aureus isolation. Results: For this, 400 cerebrospinal fluid samples were tested from hospitalized patients with arthritis in two hospitals in Urmia city during 3 months, which out of them 109 of samples were contaminated with bacteria including: 78 of isolates were Staphylococcus aureus, 12 of them were coagulase negative Staphylococci, 4 of them were Streptococcus and 15 of them were gram negative bacilli. Also, results showed that the amounts of glucose in positive samples in comparison to the amount of glucose in synovial fluid were significantly decreased. The amount of protein and the number of white blood cells in synovial fluid of positive samples were significantly higher in comparison to normal synovial fluid. Conclusion: Results showed that Staphylococcus aureus is the most common agent at infections arthritis, therefore it is recommended to use an experimental treatment for Staphylococcus aureus prior to final results.