|Evaluation of BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM|
|Shiva Pour Vahdani1, Taghi Khanzadeh2, Fattaneh Karimi3, Aylin Jahanban Esfahlan4, Somayeh Ghavipanjeh5, Amin Ghasemi1, Milad Zadi Heydarabad6, Hakim Azizi7, Akbar Darbin8, Arash Khorrami9, Ako Azimi9|
|1Student Research Committee, Maragheh University of Medical Sciences, Maragheh, Iran
2Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Community and Mental Health Nursing, School of Nursing and Midwifery, Maragheh University of Medical Sciences, Maragheh, Iran
4Department of Nursing, School of Nursing and Midwifery, Maragheh University of Medical Sciences, Maragheh, Iran
5Department of Psychiatric Nursing, Maragheh University of Medical Sciences, Maragheh, Iran
6Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
7Department of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran
8Shahid Madani Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
9Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran
CJMB 2019; 6: 209-213
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Keywords : Leukemia, Resveratrol, BCL-2 associated X, BCL-2 Gene, Apoptosis
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Objectives: Acute lymphoblastic leukemia (ALL) is considered one of the common types of cancers in childhood with an incidence of up to 25%. In addition, drug resistance is a phenomenon which reduces the chances of overcoming cancer. Further, a phytoalexin combination called resveratrol can sensitize the leukemic cells to apoptotic cell death. Due to the importance of the above-mentioned issues, the present study aimed to evaluate the effect of resveratrol on BAX and BCL-2 expression levels and apoptosis induction.
Materials and Methods: CCRF-CEM cultured cells were treated by resveratrol at doses of 15, 50, and 100 μM based on previous studies. Furthermore, RT Polymerase chain reaction (PCR) was conducted to assess the BAX and BCL-2 gene expression. Moreover, the amount of apoptosis induction was analyzed by annexin V staining method.
Results: Based on the results, time and concentration were found to play a critical role in resveratrol-induced apoptosis. Additionally, BAX upregulation and BCL-2 downregulation excreted by resveratrol in CCRF-CEM cells resulted in predisposing these cells to apoptosis.
Conclusions: In general, it was revealed that resveratrol could have a chemo-preventive activity by modifying the expression of BAX and BCL-2 genes. Finally, resveratrol was found to be a supplement drug in anti-leukemic therapy.
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