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E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Oct 2018, Vol 5, Issue 4
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Original Article
Evaluation of the BAX and BCL-2 Gene Expression Levels and Apoptosis in Resveratrol Affected Human Leukemic Cell Line: CCRF-CEM
Shiva Pour Vahdani1, Taghi Khanzadeh2, Fattaneh Karimi3, Akram Jahanban Esfahlan4, Somayeh Ghavipanjeh5, Amin Ghasemi1, Milad Zadi Heydarabad2, Hakim Azizi6, Akbar Darbin7, Arash Khorrami8, Ako Azimi8
1Student Research Committee, Maragheh University of Medical Sciences, Maragheh, Iran
2Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Community and Mental Health Nursing, School of Nursing and Midwifery, Maragheh University of Medical Sciences, Maragheh, Iran
4Department of Nursing, School of Nursing and Midwifery, Maragheh University of Medical Sciences, Maragheh, Iran
5Department of Psychiatric Nursing, Maragheh University of Medical Sciences, Maragheh, Iran
6Department of Medical Parasitology, Zabol University of Medical Sciences, Zabol, Iran
7Shahid Madani Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
8Department of Basic Sciences, Maragheh University of Medical Sciences, Maragheh, Iran


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Keywords : Leukemia, Resveratrol, BCL-2 associated X, BCL-2 Gene, Apoptosis
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Abstract

Objectives:  Acute leukemia is one of the common cancers in childhood, and in this age, up to 25% of cancer incidence belong to acute lymphoblastic leukemia. Drug Resistance is a phenomenon that has reduced the chances of  overcoming cancer.  Phytoalexin combination called resveratrol could sensitize the leukemic cells to apoptotic cell death.  We aimed to  evaluate the effect of resveratrol on BAX and BCL-2 expression levels and  induction of apoptosis.

Materials and Methods:  CCRF-CEM cultured cells were treated by resveratrol at doses of 15, 50 and 100 µM based on previous study.  RT PCR was conducted to evaluation of BAX and BCL-2 gene expression. Also, amount of apoptosis induction was analized by annexin V staining method.

Results:  Time and concentration  played a critical role in resveratrol-induced apoptosis. Presumably, BAX upregulation and BCL-2  downregulation that excreted by resveratrol in CCRF-CEM cells,  led to predisposing these cells to apoptosis.

Conclusions:  We showed that resveratrol could has a chemo-preventive activity by changing the expression of BAX and BCL-2 gene expression and can be regarded as a supplement drug, through the anti-leukemic therapy.

 

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Articles by Pour Vahdani S
Articles by Khanzadeh T
Articles by Karimi F
Articles by Ghavipanjeh S
Articles by Ghasemi A
Articles by Zadi Heydarabad M
Articles by Azizi H
Articles by Darbin A
Articles by Khorrami A
Articles by Azimi A

PubMed

Articles by Shiva Pour Vahdani
Articles by Taghi Khanzadeh
Articles by Fattaneh Karimi
Articles by Amin Ghasemi
Articles by Hakim Azizi
Articles by Akbar Darbin
Articles by Arash Khorrami
Articles by Ako Azimi

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Aras Part Medical International Press Editors in Chief
Arash Khaki
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Zafer Akan Deputy Editor
Javadi, Leila
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