|Protective Effects of Vitamin E and Selenium on Liver Tissue Damages Induced by Electromagnetic Field: An Ultrastructural Study|
|Effat Kiafar1, Hamid Tayefi Nasrabadi1, Ali Abedelahi1, Hamed Shoorei1, Abbas Majdi Seghinsara1|
|1Department of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran|
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Keywords : EMF, Liver, Oxidative stress markers, Vitamin E, Selenium
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Objectives: The aim of this study was to investigate the effect of vitamin E and selenium on some liver enzymes, some oxidative stress markers, and the liver structure of mice using light and transmission electron microscope (TEM) after exposing them to the long-term EMF.
Materials and Methods: Forty-eightfemale Balb/c mice were randomly divided into 6 groups (n=8) fallowing: control, EMF, EMF plus Vitamin E, EMF plus selenium, EMF plus vitamin E and selenium, and only vitamin E plus selenium groups. Mice in EMF group daily exposed to EMF (3 mT and 50 Hz), 4 hours for 2 months. Treated mice for 2 months intraperitoneally and daily received 0.1 mg/kg Se and/or 1.35 mg/kg vitamin E. Anesthesia in mice induced by ketamine/xylazine (80/8 mg/kg) and after taking blood samples for investigating some liver enzymes, mice killed by cervical dislocation and their liver removed to morphological studies by light and transmission electron microscope and to evaluate some oxidative stress factors.
Results: the level of malondialdehyde (MDA) increased in the liver of mice under EMF while the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx) and total antioxidant (TAC) decreased (P <0.05). Treated EMF groups by vitamin E plus Se and vitamin E alone significantly improved the levels of SOD, GPx, and TAC (P <0.05), whilst treatment with selenium did not result to a significant effect. Histopathological analysis revealed the mice’ liver that received vitamin E plus selenium and vitamin E had fewer morphological changes compared to EMF group. Moreover, some liver enzymes such as serum aminotransferase (AST), serum alanine aminotransferase (ALT) and alkaline phosphatase (ALP) significantly increased in the EMF group in comparison with other groups except for EMF group treated by Se.
Conclusion: Our results indicated although selenium singly had no significant effects, vitamin E and vitamin E + selenium significantly reduced oxidative stress and some liver enzymes.
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