|The Effects of Nanocomposite Containing Ostrich Eggshell on Calvaria Bone Formation in Rabbit: Radiographic and Hematological Survey|
|Hanieh Alemi1, Ahmad Asghari1, Gholamreza Abedi1, Abolfazl Akbarzadeh2, Pejman Mortazavi4|
|1Department of Veterinary Surgery, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Universal Scientific Education and Research Network (USERN), Tabriz, Tabriz, Iran
4Department of Veterinary Pathology, Science and Research Branch, Islamic Azad University, Tehran, Iran
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Keywords : Nanocomposite, Ostrich eggshell, Calvaria healing, Rabbit
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Objective: Available treatments to treat bone defects sometimes have limitations supply and application of the biological agents as mediators of bone regeneration stills the safe method. The aim of the current study was to determine possible effects Eggshell as agricultural waste product in the form of nanocomposite containing Ostrich Eggshell (NCOE) on calvaria bone formation in rabbit.
Materials and Methods: 15 New Zealand white adult male rabbits were used in this study. Four full-thickness skull defects were created in the calvarial bone. The first defect (control) and kept unfilled. The second defect was filled with nano material. The third defect was filled using an autogenous bone. The defect 4, was filled with mixture of nanocomposite containing Ostrich Eggshell and autogenous bone. At 30, 60 and 90 days after surgery blood samples obtained and red blood cell, white blood cell, hemoglobin, hematocrit, mean cell volume, mean cell HGB and platelets were determined. At 30, 60 and 90 days animals scarified and bone density was determined using radiography images.
Results: According to the results, significant difference observed on bone formation in control (0.18±0.01mmAlequiv) autograft (0.1±0.01mmAlequiv), NCOE (0.12±0.01mmAlequiv) and NCOE+autograft (0.07±0.01mmAlequiv), defects at day 30 post-surgery (P<0.05). Significant differences observed on done density in control (0.09±0.01mmAlequiv), autograft (0.13±0.01mmAlequiv), NCOE (0.14±0.01mmAlequiv) and NCOE+autograft (0.20±0.01mmAlequiv) at day 60 post-surgery (P<0.05). Significant differences detected on done density in control (0.15±0.01mmAlequiv), autograft (0.21±0.01mmAlequiv), NCOE (0.22±0.02mmAlequiv) and NCOE+autograft (0.29±0.01mmAlequiv) defects after 90 days (P<0.05). The NCOE+autograft had better bone formation in the all stage of the study (P<0.05). No significant effect observed on blood hematology indexes (P>0.05).
Conclusion: These results suggested NCOE+autograft has positive effects on calvaria healing in rabbit.
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