|Prevalence of human papillomavirus in oral squamous cell carcinoma patients of Tabriz, Iran|
|Leila Rahbarnia1, Safar Farajnia2, Gholamreza Bayazian3, Masoud Naderpour4, Hadi Feizi5|
|1Infectious and Tropical Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4Department of Otolaryngology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
5Young Research and Elite Club, Zanjan Branch, Islamic Azad University, Zanjan, Iran
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Keywords : Human papillomavirus, Nested PCR, oral squamous cell carcinoma, Iran
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Objectives: Oral squamous cell carcinoma (OSCC) is one of the commonest malignant neoplasms in the oral cavity. Although, human papillomavirus has been considered as one of the oral cancer risk factors, the extent of human papillomavirus (HPV) involvement in Squamous cell carcinoma (SCC) has remained unclear. The present study aimed to detect the prevalence of (HPV) in the patients with OSCC from Tabriz, northwest of Iran.
Materials and Methods: Genomic DNAs of 30normal nonsmoking cases and 30 cases with tongue SCC who undergone surgery in Tabriz, Iran were extracted and used for amplification of HPV L1 fragment using MY09/MY11and GP5/GP6 primers by Polymerase chain reaction (PCR) and nested PCR. The positive PCR products were sequenced to confirm presence of HPV.
Results: Among 30 OSCC samples analyzed, two cases (6.6% of 30) were positive for HPV in PCR performed by MY09/MY11primer, none of them showed positive result with GP5/GP6 primers. Nested PCR by MY/GP primers showed higher sensitivity with three positive cases (10% of 30) in SCC subjects.
Conclusion: according to these findings, HPV infection may not be a causative agent in tissue samples obtained from OSCC patients in Tabriz.
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