|Investigating The Exposition Rate of Regulatory Genes of Cell Cycle Phase G2/M in The Patients With Stomach Adenocarcinoma With The Meal Prescription of PUFAs Before and After Chemotherapy|
|Ali Rezazadeh1, Homayun Dolatkhah2, Ahad Mokhtarzadeh3, Saeedeh Hozhabr4|
|1Department of Biochemistry, Faculty of High Educational Institute of Rab-Rashid, East-Azerbaijan, Tabriz, I. R. IRAN
2Department of Biochemistry, Faculty of High Educational Institute of Rab-Rashid, East-Azerbaijan, Tabriz, IRAN
3Department of immunology research center, Faculty of Medicine, Tabriz University of Medical Sciences, East Azerbaijan Tabriz, IRAN
4Department of Biochemistry, Faculty of High Educational Institute of Rab-Rashid, East-Azerbaijan, Tabriz, IRAN
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Keywords : Stomach Adenocarcinoma, PUFAs, G2/M phase of cellular cycle, Cyclin, CyclinB1, CDK1
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Objectives: Cancer is one hyperactive disorder which can cause uncontrolled propagation of cells. Several reports show that Omega polyunsaturated fatty acids can prove their own antitumor effects in different cancerous cells by stopping operation of cellular cycle. So the main goal of this study is the investigation of the effect of Omega polyunsaturated fatty acids on the exposition rate of genes CDK1, CyclinB1, the cellular cycle of the stomach cancerous tissue in the under-chemotherapy patients with stomach adenocarcinoma.
Materials and methods: The present study was a double-blind clinical trial before intervention and after intervention in which neither the oncologist nor the patients were aware of the treatment. The patients with stomach adenocarcinoma were recognized for the first time and were subjected to chemotherapy. 24 patients were chance and in the lottery selected in the case and control groups. In control group treatment was performed by cis-platin with placebo and in the case group it was begun by cis-platin with the complementary capsule of fatty acids namely Natural Factors Ultimate -Omega Factors with the dose of 1200 mg made by America and 3600 mg daily, three 1200 mg medicines each course for three weeks. Three samples of stomach biopsy were taken from all patients before and after chemotherapy. Among biopsy samples, all tissues' mRNAs were extracted. Then DNA was synthesized according to them and the exposition rate of desired genes was measured using Real-Time PCR method.
Results:There was a significant decrease in the mean of exposition of genes in the case group compared to control one (p values equal to 0.021 and 0.001, respectively.
Conclusion: Obtained results of this study show that use of Omega fatty acids 3,6 and 9 with Cisplatin medicine can be effective in stopping the cell cycle phase G2 in cancerous cells of stomach tissue
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