|The Study of MTHFR Gene C677T Polymorphism in Human Embryonic Blastomeres by Fluorescence PCR Method|
|Saeedeh Saeedi1, Mohammad Reza Mashayekhi1, Masoud Maleki1|
|1Department of Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran|
CJMB 2018; 5: 248–252
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Keywords : PGD, PEP-PCR, MTHFR gene, C677T polymorphism, Recurrent miscarriage
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Objectives: The aim of the present study was to evaluate methylene tetrahydrofolate reductase (MTHFR) gene C677T polymorphism in human pre-implantation embryos.
Methods: Fifty embryos after biopsy at cleavage stage (which were inappropriate for assisted reproductive techniques) were analyzed in order to study MTHFR C677T polymorphism and sex determination. After cell lysis, in the frst step, whole genome amplifcation (WGA) was performed by primer extension pre-amplifcation (PEP) PCR method. In the next step, ﬂuorescence PCR
Results: only 37 blastomeres among 50 samples were successfully amplifed; 23 male and 14 female embryos were observed. The frequencies of CC, CT and TT genotypes in embryos were 67.56%, 24.32%, 8.10%, respectively. Additionally, the frequencies of 677C and 677T alleles were 80% and 20%, respectively.
Conclusions: In this study, the frequency of MTHFR c.677C˃T genotype was evaluated in human embryonic blastomeres before implantation. Therefore, this polymorphism may provide a new and valuable biomarker for evaluating the embryo usefulness and improving the In vitro fertilisation (IVF) process by choosing the best embryo. Our fndings revealed that the detection rate of C677T polymorphism was 74% in the samples.
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