Login Contact Us
E-ISSN : 2148-9696
Crescent Journal of
Medical and Biological Sciences
Apr 2018, Vol 5, Issue 2
Advanced Search
How do you find the scientific quality of the published articles on our web site?
Original Article
Combined Neuroprotective Action of JWH-015 and AM251 in the CA1 Hippocampal Area of Rat Model of Transient Global Cerebral Ischemia
Maryam Shiasi1, Keywan Mortezaee2, Farid Abolhassani1, Zahra Nadia Sharifi3, Marzieh Derakhshan-horeh1,4, Azim Hedayatpour1
1Department of Anatomy, School of Medicine, Tehran University of Medical Science, Tehran, Iran
2Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
3Department of Anatomy, School of Medicine, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
4Gynecology and andrology center, khanevadeh-hospital, Esfahan, Iran

Viewed : 129 times
Downloaded : times.

Keywords : Apoptosis,CA1, Survival, Global cerebral ischemia
| Related Articles
Objective: Transient global cerebral ischemia (TGCI) induces by occlusion of bilateral common carotid artery (BCCAO), and it mediates neuronal cell deathof the CA1 hippocampal area. AM251 is a cannabinoid receptors type 1 (CB1) blocker that has been known to be protective against transient focal cerebral ischemia. JWH-015 is a selective agonist of CB2 and activator of CB1 that is involved in promotion of neuronal recovery and survival. The aim of this study was to investigate the role of combined application of JWH-015 with AM251 in the rat model of GCI.

Materials and Methods: Male Wistar rats underwent 20 min of ischemia followed by reperfusion. Then, 1mg/kg JWH-015 and 2 mg/kg AM251were administered through caudal vein.The groups were control, sham, ischemia, vehicle, AM251, JWH-015 and AM251 + JWH-015.Animals were sacrificed 1 weekafter BCCAO.

Results: The AM251 + JWH-015 group indicated a significant increase in the protein expressions of AKT1, Bcl-XL, Bcl-2 and Bad 14-3-3, but it showed a considerable decrease in the protein expressions of Bad and JNK1/2 (p ≤ 0.05 vs. AM251, and JWH-015 groups).The AM251 + JWH-015 group had a significant higher number of alive cells and lower number of apoptotic cells in the CA1 hippocampal area, and it also had a considerable improvement of spatial memory (p ≤ 0.05 vs. AM251, and JWH-015 groups).

Conclusion: The results of this study indicated that combined application of AM251 and JWH-015 could be neuroprotective against detrimental effects of ischemia probably via suppression of neuronal apoptosis and maintenance of their survival.


Cite By, Google Scholar

Google Scholar

Articles by Shiasi M
Articles by Mortezaee K
Articles by Abolhassani F
Articles by Nadia Sharifi Z
Articles by Derakhshan-horeh M
Articles by Hedayatpour A


Articles by Maryam Shiasi
Articles by Keywan Mortezaee
Articles by Farid Abolhassani
Articles by Azim Hedayatpour

Submit Paper
Online Submission System
CJMB ENDNOTE ® Style Tutorials Publication Charge Women's Reproductive Health Research Center
Publication Information
Aras Part Medical International Press Editors in Chief
Arash Khaki
Zafer Akan Deputy Editor
Javadi, Leila
Published Article Statistics