|The Anticancer Effect of Arctium Lappa and Glycyrrhiza Glabra on HT-29 Colon Cancer and MCF-7 Breast Cancer Cell Lines|
|Shiva Alizadeh Nazmi1, Alireza Nourazarian2, Reza Bahhaj3, Fatemeh Khakikhatibi2,4|
|1Department of Toxicology, Ahar Branch,Islamic Azad University, Ahar, Iran
2Department of Biochemistry and Clinical Laboratories, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Parasitology and Mycology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
4Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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Keywords : Arctium, Glycyrrhiza, Colonic Neoplasm, Breast Neoplasm
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Objective: Breast and colon cancer are the most common malignancy of worldwide and common causes of mortality in Western societies. The aim of the current study examine the effectiveness of Glycrrhiza glabral and Arctium extracts on breast cancer (MCF7) and gastrointestinal cancer (HT 29) cell lines, with regard to food consumption, low cost and easy public access to in this study.
Material and Methods: The two dose and time variables were considered in this study, in order to determine the optimal effects of the drug. Two MCF-7 and HT29 cell lines were cultured in a single layer in RPMI 1640 culture medium and MTT method and flow cytometric method were used to examine apoptosis.
Results: The study of cell survival using MTT showed that the Glycrrhiza glabral aqueous extract dose of 2000 μg per ml and 24 hours of incubation had the most inhibitory effect on the MCF-7 cell line. And a dose of 2000 micrograms per ml of Glycrrhiza glabral aqueous extract with 24-hour incubation represents apoptosis in flow cytometric method.
Conclusion: The results of this study confirm the effectualness of this herbal supplement for treatment of breast cancer, and the inclusion of this herbal supplement in the diet may be effective in treating breast cancer, given the naturalness of the product, its low cost and public availability.
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