|Protective Effect of Ghrelin on Oxidative Stress and Tissue Damages of Mice Testes Followed By Chemotherapy With Cyclophosphamide|
|Ramin Salimnejad1, Jafar Soleimani Rad 1,2, Daryoush Mohammad Nejad3|
|1Department of Anatomical Science, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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Keywords : Chemotherapy, Cyclophosphamide; Oxidative stress, Ghrelin, Testes
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Objective: Cyclophosphamide is one of the common medications that used as chemotherapy and immune-suppressive in organ transplantation. Despite numerous clinical applications of this drug in cancer treatment, it causes adverse effects on body tissues, especially the male reproductive organs by increasing oxidative stress. Present study aimed to analyze the effects of ghrelin, as an antioxidant substance, on testicular damages that induced by Cyclophosphamide.
Material and Methods: Forty male mice were randomly divided into 4 groups: 1) control; 2) cyclophosphamide; 3) cyclophosphamide + ghrelin; and 4) ghrelin. Cyclophosphamide (100 mg/kg body weight) was injected intraperitoneally once a week and ghrelin (80 μg/kg body weight) were administered daily for 5 weeks. After 5 weeks, testicles were removed and we were investigated histological changes and testicular oxidative markers including malondialdehyde, superoxide dismutase, glutathione peroxidase, and total antioxidant capacity.
Results: Our results showed that cyclophosphamide increased the malondialdehyde level and decreased glutathione peroxidase, superoxide dismutase, and the total antioxidant capacity (p<0.05). Furthermore, degenerative changes in the testicular tissue were observed in cyclophosphamide group. The aforementioned factors were improved in the group that treated with ghrelin (p<0.05).
Conclusion: The results of this study revealed that ghrelin decreases the damages caused by cyclophosphamide in mice testicular tissue by reducing lipid peroxidation and increasing total antioxidant capacity.
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