|Immunogenic Properties of Sonicated Multivalent Antigen of Pseudomonas Aeruginosa|
|Sanam Rezaei Benam1, Asghar Tanomand2, Behbood Jafari1|
|1Department of Microbiology, Islamic Azad University, Ahar branch, Ahar, Iran
2Department of Microbiology, Maragheh University of Medical sciences, Maragheh, Iran
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Keywords : Pseudomonas aeruginosa, Multivalent antigen, Immunization, Vaccine candidate
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Objective: Pseudomonas aeruginosa (P. aeruginosa) is one of the opportunist pathogens that takes the advantage of patient"s immunodeficiency and is one of the major causes of nosocomial infections. Multivalent vaccines such as using the collection of secretory antigens and inactivated or killed whole-cell can be effective in preventing the infections.
Evaluation of protective effect of pseudomonas aeruginosa whole-cell antigen.
Material and Methods: In this study, sonicated multivalent antigen was provided and active immunization of female mice between 6-8 weeks (4 groups each with 7 mice) was carried out by subcutaneous, intraperitoneal and intramuscular injections on days 0, 14 and 28. The fourth group was selected as the control group of the study. One week after each injection, on days 7, 21 and 35 blood samples were taken from mice and after isolating the serum, indirect ELISA was carried out to evaluate the produced IgG antibody against sonicated multivalent antigen of P. aeruginosa.
Results: According to the results, due to the complexity of P. aeruginosa pathogenicity, using multivalent vaccines such as the collection of somatic and secretory antigens, can be effective in the prevention of infections. The Elisa results showed significantly antibody production in subcutaneous, intraperitoneal and intramuscular injections groups comparing control group. Comparing the immunization of antigen based on the route of injection showed that there was not any significant difference in induced immunization by different groups (subcutaneous, intraperitoneal and intramuscular injections).Also immunized mice showed significant protection against intraperitoneal challenge with 7.5 × 107 CFU (2× LD50) P. aeruginosa
Conclusions: The sonicated antigens can have a more effective role in immunization and the prevention of mice death against pseudomonas infections.
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