|Comparison of RNA extraction methods of breast and gastric cancer tissues|
|Seyed Amin Norollahi1, Parviz Kokhaee2,3, Ali Rashidy-Pour4,5, Vida Hojati1, Seyedeh Elham Norollahi4, Laleh Vahedi Larijani6, Ali Akbar Samadani3,4|
|1Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
2Department of Immunology,Faculty of Medicine, Semnan university of medical Sciences,Semnan, Iran
3Faculty of Medicine, Cancer Research Center,Semnan university of Medical Sciences, Semnan, Iran
4Faculty of Medicine, Research Center of Physiology, Semnan university of Medical Sciences, Semnan, Iran
5Department of Physiology, Faculty of Medicine,Semnan university of Medical Sciences, Semnan, Iran
6Department of Pathology, BoAli Sina Hospital, Mazandaran University of Medical Sciences, Sari, Iran
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Keywords : RNA extraction, Histopathological, gastric cancer, breast cancer
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Objective: Optimal quality and quantity of extracted RNA is the first step in molecular biology analysis and investigation. In this way, several methods have been proposed in order to obtain the best quality of RNA in different cases. On the other hand, RNA extraction from cells and tissues is different.
Materials and Methods: Therefore, in this study, the effects of four common RNA extraction kits including Trizol AcuZol, Ribozol and TriPure and also the effect of RNA later and liquid nitrogen were compared and studied on 50 breast cancer and 50 gastric cancer tissues. Remarkably, the quality of extracted RNA was investigated using real time PCR TaqMan assay on HER2 gene.
Results: The results showed better relative quality in Trizol kit when compared with others in this study.
Conclusion: Conspicuously, fewer amount of tissues between 10 to 30 mg lead to gain a much better quality of RNA. Meanwhile, the gene expression of HER2 gene indicates a suitable performance of extracted RNA quality and quantity. Notably, GAPDH gene was sued as internal control in all samples.
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