|The Effect of Melatonin on Izumo1 Gene Expression, Sperm Motility and In Vitro Fertilization in Mice|
|Monireh Kanani1, Jafar Soleimani Rad2, Nahid Karimian3, Alieh Ghasemzadeh1, Leila Roshangar4|
|1Women’s Reproductive Health Research Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
2Department of Tissue Engineering, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3Department of Genetic, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
4Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
CJMB 2017; 4: 139–143
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Keywords : Melatonin, In vitro fertilization, IZUMO1, Gamete fusion, RT-PCR
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Objective: IZUMO 1 is one of the most important and the most recent known proteins of sperm-oocyte fusion function. The present study aims to investigate the expression of IZUMO1 gene with melatonin injection in in-vitro conditions. Moreover, the sperm-oocyte fusion rate in in vitro fertilization (IVF) condition was examined.
Materials and methods: In this study, 30 female and 45 male mice were divided into control and experimental groups. To investigate Izomo1 gene expression and sperm motility, 10 μM melatonin was added to the culture medium in the experimental group for 1 hour and to examine the fertilization rate 10 μM melatonin was added to culture medium in the experimental group for 1 hour. Then, sperms of the control and experimental groups were added to the oocyte collected and the fertilization was examined on embryo formation.
Results: Examinations showed a significant increase in IZUMO1 gene expression and sperm motility in the experimental group (receiving melatonin) compared to the control group. Counting formed embryos showed that 83% of the oocytes have evolved to the fetus.
Conclusion: We conclude that melatonin may be a good alternative for rising oocyte fertilization success by sperm in IVF.
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